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anti smad3 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti smad3 antibody
    Anti Smad3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+smad3+antibody/Smad3+Rabbit+mAb/10__1186_slash_s10020___026___01638___7-189-7-10
    Average 96 stars, based on 1113 article reviews
    anti smad3 antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.

    Staining:

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.

    Incubation:

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.

    Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3
    Article Snippet: Lysis buffer (50 mM Tris base, 150 mM NaCl, 2 mM EDTA, 1% NP40), supplemented with protease and phosphatase inhibitors (NCM Biotech, China, #P002), was added to CRC cells, and protein samples were obtained by centrifugation to remove insoluble precipitates. .. Protein A/G magnetic beads were incubated with anti-Flag (CST, #14793S), anti-SMAD3 antibody (CST, #9523S, 1:100), anti-RACK1 antibody (CST, #5432S, 1:100), anti-His antibody (Proteintech, #66,005–1-Ig, 1:1000), or IgG (Beyotime, #A7058) for 4 h at 4 °C on a rotary shaker. ..

    Article Title: Forsythiaside A inhibits the progression of intrahepatic cholangiocarcinoma partially via suppressing oxidative stress-induced hepatic stellate cell activation and macrophage polarization
    Article Snippet: Equal amount of protein (20 μg) of each sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and then transferred onto polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). .. The membranes were blocked with 5% skimmed milk at room temperature for 2 h. Subsequently, the membranes were incubated with anti-Wnt family member 5a (Wnt5a) antibody (1:1000; ab325179, Abcam, Shanghai, China) and anti-α-smooth muscle actin (α-SMA) antibody (1:1000; ab124964, Abcam), anti-collagen type I α 1 chain (COL1A1) antibody (1:1000; ab260043, Abcam), anti-peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) antibody (1:1000; ab191838, Abcam), anti-mitochondrial transcription factor a (TFAM) antibody (1:1000; ab307302, Abcam), anti-dynamin-related protein 1 (DRP1) antibody (1:1000; ab184247, Abcam), anti-mitofusin 2 (MFN2) antibody (1:1000; ab124773, Abcam), anti-optic atrophy protein 1 (OPA1) antibody (1:1000; ab157457, Abcam), anti-CD200 receptor (CD200R) antibody (1:1000; PA5-117704, Thermo Fisher Scientific), anti-C-C motif chemokine ligand 22 (CCL22) antibody (1:1000; 500-P107-1MG, Thermo Fisher Scientific), anti-protein kinase B (AKT1) antibody (1:1000; #2938, Cell signaling technology, Shanghai, China), anti-phosphorylated (p)-AKT1 antibody (1:1000; #4060, Cell signaling technology), anti-Smad2 antibody (1:1000; #5339, Cell signaling technology), anti-p-Smad2 antibody (1:1000; #18338, Cell signaling technology), anti-Smad3 antibody (1:1000; #9523, Cell signaling technology), anti-p-Smad3 antibody (1:1000; #9520, Cell signaling technology), anti-hypoxia-inducible factor 1-α subunit (HIF-1α) antibody (1:1000; #14179, Cell signaling technology), and anti-GAPDH antibody (1:1000; ab181602, Abcam), respectively, overnight at 4 °C. .. After washing three times with Tris Buffered Saline Tween (TBST), the membranes were incubated with the corresponding secondary antibody (1:5000; ab6721, Abcam) at room temperature for 2 h. The protein bands were detected using the BeyoECL Plus kit (Beyotime), and the band density was quantified using the Quantity One densitometer software.

    Article Title: PSPC1-SMAD3 axis regulates iron-induced beiging of adipocytes in white adipose tissue
    Article Snippet: Cells were lysed with NP-40 lysis buffer (Beyotime, China) and centrifuged at 12,000 g at 4 °C for 15 min to collect the supernatant protein. .. Antibodies including anti-PSPC1 antibody (Santa Cruz Biotechnology, sc374181), anti-SMAD3 antibody (CST, #9523), control IgG antibody (Abclone, AC005, AC011) were incubated with pre-washed protein A/G magnetic beads (MCE, HY-K0202) in coupling buffer at 4 °C for 2 h for covalent crosslinking. ..

    Magnetic Beads:

    Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3
    Article Snippet: Lysis buffer (50 mM Tris base, 150 mM NaCl, 2 mM EDTA, 1% NP40), supplemented with protease and phosphatase inhibitors (NCM Biotech, China, #P002), was added to CRC cells, and protein samples were obtained by centrifugation to remove insoluble precipitates. .. Protein A/G magnetic beads were incubated with anti-Flag (CST, #14793S), anti-SMAD3 antibody (CST, #9523S, 1:100), anti-RACK1 antibody (CST, #5432S, 1:100), anti-His antibody (Proteintech, #66,005–1-Ig, 1:1000), or IgG (Beyotime, #A7058) for 4 h at 4 °C on a rotary shaker. ..

    Article Title: PSPC1-SMAD3 axis regulates iron-induced beiging of adipocytes in white adipose tissue
    Article Snippet: Cells were lysed with NP-40 lysis buffer (Beyotime, China) and centrifuged at 12,000 g at 4 °C for 15 min to collect the supernatant protein. .. Antibodies including anti-PSPC1 antibody (Santa Cruz Biotechnology, sc374181), anti-SMAD3 antibody (CST, #9523), control IgG antibody (Abclone, AC005, AC011) were incubated with pre-washed protein A/G magnetic beads (MCE, HY-K0202) in coupling buffer at 4 °C for 2 h for covalent crosslinking. ..

    other:

    Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS mice injected with HCT116 mock and HCT116-shRACK1 cells were selected after tail vein injection and treated with BCI-121 (Selleck, #432529-82-3) at a dose of 5 mg/kg via peritoneal injection (i.p.) three times per week for five weeks.

    Article Title: PSPC1-SMAD3 axis regulates iron-induced beiging of adipocytes in white adipose tissue.
    Article Snippet: After blocking with 5% BSA for 30 min, SMAD3 antibodies (CST, #9523) was stained and incubated overnight at 4°C.

    Immunoprecipitation:

    Article Title: USP7-mediated stabilization of Snail1 drives macrophage-to-myofibroblast transition and promotes postoperative peritoneal adhesions
    Article Snippet: .. The sheared chromatin was immunoprecipitated using an anti-Smad3 antibody (9523S, CST, Danvers, USA) or control IgG antibody. ..

    Control:

    Article Title: USP7-mediated stabilization of Snail1 drives macrophage-to-myofibroblast transition and promotes postoperative peritoneal adhesions
    Article Snippet: .. The sheared chromatin was immunoprecipitated using an anti-Smad3 antibody (9523S, CST, Danvers, USA) or control IgG antibody. ..

    Article Title: PSPC1-SMAD3 axis regulates iron-induced beiging of adipocytes in white adipose tissue
    Article Snippet: Cells were lysed with NP-40 lysis buffer (Beyotime, China) and centrifuged at 12,000 g at 4 °C for 15 min to collect the supernatant protein. .. Antibodies including anti-PSPC1 antibody (Santa Cruz Biotechnology, sc374181), anti-SMAD3 antibody (CST, #9523), control IgG antibody (Abclone, AC005, AC011) were incubated with pre-washed protein A/G magnetic beads (MCE, HY-K0202) in coupling buffer at 4 °C for 2 h for covalent crosslinking. ..



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